Although the base sequence of most human DNA is identical from one person to another, there are sufficient differences to enable use of this information to identify individuals. These differences in the sequence of bases in DNA from one individual to another lead to differences in the distribution and frequency of restriction enzyme recognition sites. Thus, when an individual’s DNA is fragmented with a specific restriction enzyme, the number and length of the resulting fragments is unique (unless the individual has an identical twin). The different length DNA fragments are calledrestriction fragment length polymorphisms, orRFLPs(pronounced Rf-lips) for short.
After studying Figure 1, answer the following questions.
1)How many RFLPs are generated from homologue M using this restriction enzyme? from homologue P?
2)Explain this difference.
3)Are the DNA segments in the diagram an entire chromosome? an entire gene? Explain.
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